Ability of kallikrein to generate angiotensin II-like pressor substance and a proposed ‘kinin–tensin enzyme system’

Abstract

Pig pancreatic kallikrein liberates kallidin from kininogen, whereas trypsin releases bradykinin. Recently, both kallikrein and trypsin have been reported to convert inactive plasma renin to active renin. In this experiment at pH 6.0, trypsin generated an angiotensin II-like pressor substance from human plasma protein in the absence of converting enzyme. This was isolated and has the same amino acid composition as angiotensin II. Thus, in vitro trypsin can directly liberate both the depressor, bradykinin, in weak alkaline conditions, and the pressor, angiotensin II, at weakly acidic pH, from the appropriate substrates. It was investigated whether kallikrein-a serine protease like trypsin-also generates a pressor substance at weakly acidic pH. The results demonstrate that it does. Kallikrein may be involved in a direct link between the pressor and depressor systems and the term, kinin-tensin system, was proposed for this sort of one-enzyme system capable of generating both depressor and pressor substances.