A Model for Predicting Ionic Equilibrium Concentrations in Cell Walls
Open Access
- 1 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 68 (2), 415-419
- https://doi.org/10.1104/pp.68.2.415
Abstract
Purified cell walls were prepared from roots of Horse bean (Vicia faba L., var. minor) and Yellow Lupine (Lupinus luteus L.). Two methods were used: (a) grinding and (b) lysis of the endocellular contents by Triton X-100. The Ca2+, Mg2+, and K+ contents were determined after incubation in various solutions in such a manner that the measurements gave access to the undisturbed equilibrium contents. The results were used to test a model which describes the ionic atmosphere in the cell walls as a result of simultaneous electrostatic interactions between free ions (Donnan effect) and specific association equilibria, including acido-basic ones. This model correctly accounted for the whole set of experimental results and predicted the values of the unmeasurable local concentrations and pH.Keywords
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