Analysis of the Klebsiella pneumoniae ntrB gene by site-directed in vitro mutagenesis
- 1 September 1987
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 1 (2), 133-142
- https://doi.org/10.1111/j.1365-2958.1987.tb00505.x
Abstract
A number of in-frame insertion and deletion mutations have been constructed in vitro in the Klebsiella pneumoniae ntrB gene and the effects of each mutant NtrB protein on NtrC activity have been assessed after reintroduction of the ntrB mutation into the glnA ntrBC operon. These experiments suggest that the phosphorylation of NtrC catalysed by NtrB not only makes NtrC competent as a transcriptional activator but also improves the DNA-binding properties and hence the negative control functions of NtrC. The variety of NtrB phenotypes obtained suggest a structure/function model for the protein.Keywords
This publication has 24 references indexed in Scilit:
- Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate regulon of Escherichia coliJournal of Molecular Biology, 1986
- Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.Proceedings of the National Academy of Sciences, 1986
- Interaction of purified NtrC protein with nitrogen regulated promoters from Klebsiella pneumoniaeMolecular Genetics and Genomics, 1985
- Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.Proceedings of the National Academy of Sciences, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Improved plasmid vectors for the isolation of translational lac gene fusionsGene, 1984
- In vitro insertional mutagenesis with a selectable DNA fragmentGene, 1984
- Positive control and autogenous regulation of the nifLA promoter in Klebsiella pneumoniaeNature, 1983
- GENETIC CONTROL OF NITROGEN ASSIMILATION IN BACTERIAAnnual Review of Genetics, 1982
- Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene clusterMolecular Genetics and Genomics, 1982