Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy
- 1 April 1996
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 182 (1), 40-45
- https://doi.org/10.1111/j.1365-2818.1996.tb04795.x
Abstract
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.Keywords
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