Reversed Phase Ion-Pair Chromatographic Separation of Acetyl Choline and Choline
- 1 March 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (3), 537-544
- https://doi.org/10.1080/01483918508067099
Abstract
A reversed phase High-Performance Liquid Chromatographic procedure for the separation of choline and acetyl choline is described. The method employs a mobile phase containing aromatic sulphonates as counter ions for ion-pair chromatography and for U.V. Visualisation of choline and acetyl choline at 254 nm. The method enables the separation of the two compounds in the 1-10 ug concentration range in six minutes. The separations are better achieved on a PRP-I resin column.This publication has 7 references indexed in Scilit:
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