LexA-binding sequences in Gram-positive and cyanobacteria are closely related
- 2 December 2003
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 271 (1), 40-49
- https://doi.org/10.1007/s00438-003-0952-x
Abstract
The lexA gene of the cyanobacterium Anabaena sp. strain PCC7120 has been cloned by PCR amplification with primers designed after TBLASTN analysis of its genome sequence using the Escherichia coli LexA sequence as a probe. After over-expression in E. coli and subsequent purification, footprinting experiments demonstrated that the Anabaena LexA protein binds to the sequence TAGTACTAATGTTCTA, which is found upstream of its own coding gene. Directed mutagenesis and sequence comparison of promoters of other Anabaena genes, as well as those of several cyanobacteria, allowed us to define the motif RGTACNNNDGTWCB as the LexA box in this bacterial phylum. Substitution of a single nucleotide in this motif present in the Anabena lexA promoter is sufficient to enable it to bind the Bacillus subtilis LexA protein. These data indicate that Cyanobacteria and Gram-positive bacteria are phylogenetically closely related.This publication has 32 references indexed in Scilit:
- Geobacter sulfurreducensHas Two AutoregulatedlexAGenes Whose Products Do Not Bind therecAPromoter: Differing Responses oflexAandrecAto DNA DamageJournal of Bacteriology, 2003
- A Green Nonsulfur Bacterium,Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive OrganismsJournal of Bacteriology, 2002
- Quantification of RecA protein in Deinococcus radiodurans reveals involvement of RecA, but not LexA, in its regulationMolecular Genetics and Genomics, 2002
- Definition of the Mycobacterial SOS Box and Use To Identify LexA-Regulated Genes inMycobacterium tuberculosisJournal of Bacteriology, 2002
- The LexA Protein from Deinococcus radiodurans Is Not Involved in RecA Induction following γ IrradiationJournal of Bacteriology, 2001
- Crystal Structure of LexA: A Conformational Switch for Regulation of Self-CleavageCell, 2001
- Dual Repression by Fe 2+ -Fur and Mn 2+ -MntR of the mntH Gene, Encoding an NRAMP-Like Mn 2+ Transporter in Escherichia coliJournal of Bacteriology, 2001
- A model for the LexA repressor DNA complexProteins-Structure Function and Bioinformatics, 1995
- Nature of the SOS-inducing signal in Escherichia coliJournal of Molecular Biology, 1990
- Molecular Cloning and Isolation of a Cyanobacterial Gene Which Increases the UV and Methyl Methanesulphonate Survival of recA Strains of Escherichia coli K12Microbiology, 1987