Culture of early somite mouse embryos during organogenesis
Open Access
- 1 January 1979
- journal article
- research article
- Published by The Company of Biologists in Development
- Vol. 49 (1), 17-25
- https://doi.org/10.1242/dev.49.1.17
Abstract
Early somite (2-4) mouse embryos were explanted and then maintained in culture for 24 or 48 h intervals. Various types of media were tested and it was determined that rat serum supported normal growth over a period of 48 h, based on total protein analysis and histological comparisons with in vivo specimens. Other media including fetal calf serum and fetal calf serum + Waymouth’s (1:1) supported some growth, but did not equal the success of using rat serum alone. During the 48 h culture period in rat serum, embryos developed to stages indistinguishable from embryos maintained for a similar time in vivo.This publication has 10 references indexed in Scilit:
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