A Simple Enzyme-Immunoassay (EIA) Test for Factor VIII-Related Antigen (VIIIAGN)

Abstract

An enzyme immunoassay (EIA) system has been developed to measure factor VIII- related antigen (VIII_AGN). This assay gives similar results to the commonly used Laurell electroimmunodiffusion (EID) assay for VIII_AGN as shown by comparison of both techniques with samples from healthy controls, patients with hemophilia A, and patients with von Willebrand’s disease. The assay also has a greater precision than the EID technique as demonstrated by multiple assays of aliquots of a single sample. The use of this EIA test for VIII_AGN is simple and employs inexpensive reagents and equipment. The use of expensive antisera is minimized. EIA for VIII_AGN has the advantage of increased sensitivity compared to Laurell EIA.