Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.
- 1 March 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (6), 1950-1954
- https://doi.org/10.1073/pnas.79.6.1950
Abstract
Cloned c[complementary]DNA sequences of the murine hypoxanthine/guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) gene were isolated by using a mouse neuroblastoma cell line containing increased levels of a variant HPRT protein. These sequences were used as probes to demonstrate that protein over-production in this cell line is a consequence of at least a 20-fold increase in HPRT mRNA levels resulting from approximately 50-fold amplification of HPRT genomic sequences. The largest cDNA insert so far characterized represents about 70% of the HPRT mRNA sequence. This cDNA possesses regions of homology with mRNA and DNA from Chinese hamster, baboon and human, thus facilitating detailed analysis of this locus in these 4 spp.This publication has 30 references indexed in Scilit:
- Recovery of DNA fragments inserted by the “tailing” method: Regeneration of PstI restriction sitesGene, 1981
- Overlap hybridization screening: Isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome IIIGene, 1979
- The HPRT locusCell, 1979
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979
- Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsGene, 1979
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- The isolation of a suppressible nonsense mutant in mammalian cellsCell, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: Evidence for mutations at different loci in the HGPRT geneJournal of Cellular Physiology, 1975
- Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activityBiochemical Genetics, 1972