Localization of the Restrictive Event of EMC Virus Replication in Semi-permissive Monkey and Monkey-Mouse Hybrid Cells

Abstract

Encephalomyocarditis (EMC) virus replication was investigated in permissive mouse MKS cells, semi-permissive monkey CV1 cells, and in somatic monkey-mouse MKCVIII hybrid cells whose permissiveness is under the negative control of the simian genome. In CV1 cells the synthesis of single- and double-stranded virus RNA was restricted. In semi-permissive hybrid Cl4/3 cells only the single-stranded virus RNA was synthesized in small amounts, but the double-stranded virus RNA accumulated late after infection. The synthesis of virus polyribosomes and virus polypeptides was lowered in semi-permissive conditions. In the presence of quaternary NH4+, the synthesis of EMC virus was partially relieved in CV1 cells. Thus, a defective function in the replication complex is apparently involved in the restrictive event.