Abstract
Staphylococci, when grown on a medium free from riboflavin, synthesize a fluorescent substance which seems to be riboflavin, as shown by the lumiflavin test and the lactobacillus growth test. This is true of Staphylococcus aureus when grown in a relatively simple non-synthetic medium, and also of Staph. flavus when grown in a synthetic medium containing only glucose and inorganic salts.