A Single Amino Acid Substitution in the ICP27 Protein of Herpes Simplex Virus Type 1 Is Responsible for Its Resistance to Leptomycin B
Open Access
- 15 January 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (2), 1039-1043
- https://doi.org/10.1128/jvi.75.2.1039-1043.2001
Abstract
Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.Keywords
This publication has 26 references indexed in Scilit:
- CRM1 Mediates Nuclear Export of Nonstructural Protein 2 from Parvovirus Minute Virus of MiceBiochemical and Biophysical Research Communications, 1999
- The Specificity of the CRM1-Rev Nuclear Export Signal Interaction Is Mediated by RanGTPJournal of Biological Chemistry, 1998
- Leptomycin B Inhibition of Signal-Mediated Nuclear Export by Direct Binding to CRM1Experimental Cell Research, 1998
- Posttranscriptional Regulation in Herpes Simplex VirusSeminars in Virology, 1998
- The Herpes Simplex Virus Immediate-Early Protein ICP27 Shuttles between Nucleus and CytoplasmVirology, 1998
- The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm.Genes & Development, 1994
- Shuttling of pre-mRNA binding proteins between nucleus and cytoplasmNature, 1992
- Leptomycins A and B, new antifungal antibiotics. I. Taxonomy of the producing strain and their fermentation, purification and characterization.The Journal of Antibiotics, 1983
- Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genomeCell, 1977
- Inhibition of Herpes Simplex Virus Replication by Phosphonoacetic AcidAntimicrobial Agents and Chemotherapy, 1974