HUMAN-LYMPHOCYTE MARKERS DEFINED BY ANTIBODIES DERIVED FROM SOMATIC-CELL HYBRIDS .1. HYBRIDOMA SECRETING ANTIBODY AGAINST A MARKER SPECIFIC FOR HUMAN LYMPHOCYTES-B

Abstract

A hybridoma has been isolated from the products of fusion of a myeloma cell line with spleen cells from mice immunized with a human B [bone marrow-derived] cell line. After cloning, the hybridoma secretes antibody with the following properties. Human B-lymphoblastoid cell lines react with the antibody while T [thymus-derived] and null cell lines do not. The antibody reacts with the majority of leukocytes in the blood of patients with CLL [chronic lymphocytic leukemia], but with a minority of cells in the blood of patients with AML or ALL of the null or T type. The antibody reacts with 9-21% of mononuclear cells in normal peripheral blood. The reacting cells are not T cells and overlap extensively with cells identified as B cells by other markers. The antigen identified by this antibody appears to be distinct from known B cell markers, and is presented as a new B cell marker with diagnostic potential.