Expression of human α-globin genes in hybrid mouse erythroleukaemia cells depends on differentiated state of human donor cell
- 1 May 1980
- journal article
- Published by Springer Nature in Nature
- Vol. 285 (5759), 36-38
- https://doi.org/10.1038/285036a0
Abstract
We have developed a system which can be used to study the mechanisms that may govern the expression of human alpha-globin genes in human erythroid and non-erythroid haematopoietic cells. Human chromosome 16, which has been shown to bear the human alpha-globin genes, is introduced by cell fusion into mouse erythroleukemia (MEL) cells to generate continuously proliferating cell lines that retain permanently the human alpha-globin genes. We have shown that hybrid diploid MEL cells with human alpha-globin genes from erythroid donor cells express these genes fully through globin chain synthesis, while hybrid diploid MEL cells containing human alpha-globin genes from non-erythroid human haematopoietic donor cells contain very low levels of human alpha-globin mRNA and no detectable human alpha-globin chains. The levels of human alpha-globin mRNA in these hybrid cells were found to depend on factors present in the MEL recipient cell as well as on the differentiated state of the human donor cell, suggesting that this system may be suitable for characterisation of mechanisms governing haematopoietic differentiation in man.Keywords
This publication has 17 references indexed in Scilit:
- Human α-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cellsCell, 1978
- Globin RNA sequences in human leukaemic peripheral bloodNature, 1978
- Active conformation of the globin genes in uninduced and induced mouse erythroleukemia cellsCell, 1978
- Localization of the human α-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assayCell, 1977
- A sequential staining technique for the chromosomal analysis of interspecific mouse/hamster and mouse/human somatic cell hybridsExperimental Cell Research, 1977
- Local theory of Fuchsian systems, IProceedings of the Japan Academy, Series A, Mathematical Sciences, 1975
- The diversity of the messenger RNA population in growing friend cellsCell Differentiation, 1974
- Globin messenger RNA synthesis and processing during haemoglobin induction in friend cells. I. Evidence for transcriptional control in clone M2Cell Differentiation, 1974
- Clinical PhysicsScandinavian Journal of Clinical and Laboratory Investigation, 1968
- Abnormal human haemoglobins: Separation and characterization of the α and β chains by chromatography, and the determination of two new variants, Hb chesapeake and Hb J (Bangkok)Journal of Molecular Biology, 1966