Cloning, sequencing and expression of a full‐length rabbit fast skeletal troponin‐C cDNA
Open Access
- 8 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (1), 22-26
- https://doi.org/10.1016/0014-5793(88)80576-3
Abstract
A full‐length cDNA coding for troponin‐C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed protein was found to be identical to purified rabbit skeletal troponin‐C based on electrophoretic mobilities in polyacrylamide gels containing either SDS or urea, and on immunoblotting. These results establish that our troponin‐C cDNA clone is suitable for site‐directed mutagenesis studies on the structure and function of troponin‐CKeywords
This publication has 22 references indexed in Scilit:
- Structural Aspects of Troponin-Tropomyosin Regulation of Skeletal Muscle ContractionAnnual Review of Biophysics, 1987
- TRANSCRIPTION TERMINATION AND THE REGULATION OF GENE EXPRESSIONAnnual Review of Biochemistry, 1986
- Thin Filament Proteins and Thin Filament-Linked Regulation of Vertebrate Muscle ContractioCritical Reviews in Biochemistry, 1984
- A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun sequencingNature, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Calcium and muscle contractionProgress in Biophysics and Molecular Biology, 1968