Intragenic variation by site-specific recombination in the cryptic plasmid of Neisseria gonorrhoeae
Open Access
- 1 July 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 167 (1), 231-237
- https://doi.org/10.1128/jb.167.1.231-237.1986
Abstract
Cryptic plasmid DNA of Neisseria gonorrhoeae was found integrated into the gonococcal chromosome in both plasmid-bearing strains and plasmid-free strains. At several chromosomal locations only segments of the plasmid were found. However, in at least two strains an intact copy of the plasmid seemed to be present with the joints between the plasmid and the chromosomal DNA being located within the cppB gene of the cryptic plasmid. The cppB gene was shown to undergo a sequence-specific intragenic deletion. The deletion removed 54 base pairs, representing 18 amino acids, and did not affect the reading frame. It is proposed that the cryptic plasmid integrates into the chromosome and other gonococcal plasmids within this site-specific deletion region. Models for the site-specific recombination are presented.This publication has 49 references indexed in Scilit:
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