Isolation and preliminary characterization of temperature-sensitive mutants of Newcastle disease virus
- 1 June 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 18 (3), 848-855
- https://doi.org/10.1128/jvi.18.3.848-855.1976
Abstract
Temperature-sensitive (ts) mutants of Newcastle disease virus were isolated [grown on chicken embryo cell cultures] and characterized genetically (complementation), biochemically (RNA synthesis) and biologically (fusion from within and hemadsorption). Fifteen of these mutants were divided into 5 complementation groups. Groups A (5 mutants) and E (1 mutant) are ts for RNA synthesis (RNA-) as well as for the other functions. Group B contains 4 RNA+ mutants of which 1 is ts for fusion, 1 for hemadsorption and 2 for neither function. Group C contains 1 RNA+ mutant which is a poor cell fuser. Group D contains 2 RNA+ mutants which are ts for fusion. In addition, 2 noncomplementing mutants (group BC) fail to complement both group B and group C mutants while exhibiting complementation with mutants in groups A, D and E.This publication has 29 references indexed in Scilit:
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