Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis

Abstract

1. Young rats were offered to appetite a semi-synthetic diet either alone or containing the β₂-selective agonist clenbuterol (4-amino-α[t-butylamino) methyl]-3, 5-dichlorobenzyl alcohol).3. Male rats (starting weight 53 g) received clenbuterol at a daily oral dose of 200 pg/kg body-weight per d. Animals were slaughtered after 0, 4, 8, 11, 18, 21 and 25 d of treatment. At 4, 11, 21 and 25 d muscle protein synthesis was measured by the method of Garlicket al.(1980). Although clenbuterol increased the rate of protein and RNA accretion in gastrocnemius and soleus muscles, protein synthesis was not increased.4. The results suggested that the drug had a rapid, perhaps direct, inhibitory effect on protein degradation. It is concluded that the growth-promoting effect of clenbuterol may be specific to muscle and that the drug may act in a novel manner which circumvents the physiological mechanisms responsible for the control of muscle growth.