Muscarinic activity of McN-A-343 and its value in muscarinic receptor classification

Abstract

1 The affinity and potency of McN-A-343 (4-(m-chlorophenyl-carbamoyloxy) −2-butynyltrimethylammonium chloride) has been assessed at a range of M₁ and M₂ muscarinic receptors. McN-A-343 was shown to act as a full agonist at M₂ receptors present in the guinea-pig isolated taenia caeci (–log EC₅₀ = 5.14). McN-A-343 exhibited no agonist action in the guinea-pig ileum, atria, bladder or trachea. 2 McN-A-343 was not selective in terms of affinity since its dissociation constants at M₁ and M₂ binding sites in the rat cerebral cortex and myocardium respectively, were very similar (cortical pPKi = 5.05; myocardial pKi = 5.22). The selectivity previously reported for the compound may be due to differences in intrinsic efficacy and/or tissue receptor reserve. 3 Based on differential antagonist affinities, the muscarinic receptor profile of the taenia caeci, trachea and bladder was similar to that observed in the ileum, but dissimiliar to that observed in the atria.