Bombesin stimulates growth of human prostatic cancer cellsin vitro
- 1 May 1990
- Vol. 63 (9), 1714-1720
- https://doi.org/10.1002/1097-0142(19900501)63:9<1714::aid-cncr2820630912>3.0.co;2-h
Abstract
Cell proliferation of the human prostatic carcinoma cell line PC3 and of the epithelial cell strain PMU 23 derived from a primary culture of a stage III prostatic carcinoma was enhanced dose dependently by adding 0.1 nM to 10.0 nM bombesin (BMBS) to the culture medium. The growth stimulation was specifically inhibited by antibodies versus Gastrin Releasing Peptide (GRP) crossreacting with BMBS. Presence of BMBS-positive neuroendocrine cells in human prostate and measurable amounts of BMBS-like peptides in prostatic fluid were reported previously. In a binding assay using 125I-GRP, it was possible to demonstrate the presence of saturable specific receptors on PC3 cells, numerically comparable with those measured on small cell lung cancer cell lines. By immunofluorescence, however, no BMBS immunoreactivity on PC3 cells could be demonstrated. These observations suggest that BMBS plays a role in prostatic epithelium growth and that prostatic carcinoma may have an autocrine or paracrine proliferation stimulus within the gland microenvironment.This publication has 26 references indexed in Scilit:
- Bombesin-like immunoreactivity in human seminal fluidRegulatory Peptides, 1987
- Organization of cytokeratin bundles by desmosomes in rat mammary cells.The Journal of cell biology, 1986
- Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancerNature, 1985
- Gastrin releasing peptide is a selective mitogen for small cell lung carcinoma in vitro.JCI Insight, 1985
- Human Prostatic Carcinoma in Tissue Culture: Correlations between Histological Diagnosis and in vitro ParametersEuropean Urology, 1985
- Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.Proceedings of the National Academy of Sciences, 1983
- Dose-response comparisons of canine plasma gastroenteropancreatic hormone responses to bombesin and the porcine gastrin-releasing peptide (GRP)Regulatory Peptides, 1983
- Characterization of a gastrin releasing peptide from porcine non-antral gastric tissueBiochemical and Biophysical Research Communications, 1979
- Bombesin-like immunoreactivity in the lungNature, 1978
- Isolation and structure of bombesin and alytesin, two analogous active peptides from the skin of the european amphibiansBombina andAlytesCellular and Molecular Life Sciences, 1971