Seeds of non‐nodulating soybean (Glycine max (L.) Merrill) genotype A62‐8 were mixed in a 1:1 ratio with seeds of nodulating genotypes ‘Delmar’ and ‘Scott’. Prior to planting, the mixtures were inoculated with 7 different strains or combinations of strains of Rhizobium japonicum. A62‐8 mixed with Delmar yielded significantly more with certain inoculum treatments than when the mixture was uninoculated. However, when mixed with Scott the yields of A62‐8 did not differ with inoculum treatments. The seed protein of A62‐8 was higher when the mixtures were inoculated than when uninoculated. The yield and protein percentage of inoculated Delmar and Scott were also significantly higher than the uninoculated checks. The amount of nitrogen fixed in each mixture‐inoculum combination was estimated by using yield and protein data from each genotype.