Conformational analysis of oligoarabinonucleotides. An NMR and CD study
Open Access
- 1 January 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (13), 4583-4600
- https://doi.org/10.1093/nar/11.13.4583
Abstract
A 500 and 300 MHz proton NMR study of the series of oligoarabinonucleotides 5′aAMP, 3′aAMP, aA-aA, (aA-)2aA and (aA-)3-aA is presented. In addition, circular dichroism is used to study the stacking behaviour of aA-aA. The complete 1H-NMR spectral assignment of the compounds (except the tetramer) is given. Proton-proton and proton-phosphorus coupling constants, obtained by computer simulation of the high-field region of the spectra, yield information on the conformation of the arabinose rings (N- or S-type) and on the intramolecular stacking properties of the dimer and the trimer. The monomers 5′aAMP and 3′aAMP exhibit a preference for N- and S-type sugar conformation, respectively. It is shown that the dimer aA-aA at low temperature prefers a mixed stacked state of the type aA(S)-aA(N). In the trimer the aA(2)-aA(3) fragment exhibits a conformation similar to that found in the dimer, whereas the aA(l) residue prefers to adopt S-type sugar and has some tendency to stack upon residue aA(2).Keywords
This publication has 14 references indexed in Scilit:
- Conformational Analysis of the Nucleotides A2'-5'A, A2'-5'A2'-5'A and A2'-5'U from Nuclear Magnetic Resonance and Circular Dichroism StudiesEuropean Journal of Biochemistry, 1981
- Conformational Characteristics of the Trinucleoside Diphosphate dApdApdA and Its Constituents from Nuclear Magnetic Resonance and Circular Dichroism StudiesEuropean Journal of Biochemistry, 1980
- Conformational analysis of arabinonucleosides and nucleotides A comparison with the ribonucleosides and nucleotidesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- Correlations of conformational parameters and equilibrium conformational states in a variety of β-d-arabinonucleosides and their analoguesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- Conformation in aqueous medium of the neutral, protonated and anionic forms of 9-β-d-arabinofuranosyladenineBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Conformational analysis of the sugar ring in nucleosides and nucleotides. Improved method for the interpretation of proton magnetic resonance coupling constantsJournal of the American Chemical Society, 1973
- Conformational analysis of the sugar ring in nucleosides and nucleotides. New description using the concept of pseudorotationJournal of the American Chemical Society, 1972
- Conformation of the exocyclic 5′-CH2OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H5′, and H5″ signals in the NMR spectraBiochemical and Biophysical Research Communications, 1972
- Interactions contributing to the stability of a polynucleotide helical chain role of the 2′-hydroxyl and of the phosphate groupsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Comparison of Conformational Characteristics of Arabinose and Ribose containing Dinucleoside PhosphatesNature, 1968