Purification ofEscherichia coli30S Ribosomal Proteins by High Performance Liquid Chromatography

Abstract
High performance liquid chromatography was applied to the separation of proteins derived from the E. coli 30S ribosomal subunit. Several methods of separating this protein mixture were tested: size-exclusion chromatography on hydrophilic phases; ion exchange and reversed phase chromatography (on C2-C18 hydrocarbon-bonded supports). Various elution systems were examined to obtain pure proteins suitable for micro-sequence analysis. The resolution and yields of the proteins varied considerably, depending on the type of support and gradient system used. The best results were achieved with uniformly globular-shaped supports of large pore size, and by combining high performance size exclusion with rechromatography on reversed phase columns. Purification conditions for the individual proteins are listed. The methods employed avoid any precipitation step and allow easy identification of the proteins by 1- or 2-dimensional gel electrophoresis, amino-acid analysis or direct manual or automatic micro-sequencing. Since the isolation time is much reduced compared with conventional purification procedures, the proteins obtained by these techniques are well suited for topographical and immunological studies or reconstitution assays. Ribosomal proteins of other organisms can be separated under similar conditions.

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