EFFECTS OF PRESERVATION CONDITIONS AND TEMPERATURE ON TISSUE ACIDIFICATION IN CANINE KIDNEYS

Abstract

The influence of temperature (5, 15, 25, and 35$dGC) on the degree of tissue acidification was examined using 74 canine kidneys with simple ischemia or after protection of the kidneys with Euro-Collins solution or with the HTK-solution of Bretschneider. At an incubation temperature of 5$dGC, the intrarenal pH value in HTK-protected kidneys is continuously higher than 7.3 during 36 hr of ischemia. In Euro-Collins kidneys the pH value decreases to a pH of 6.4 during this time. In simple ischemic kidneys pH is 6.3 after 36 hr. At 15$dGC the pH value falls to a lower level in Euro-Collins kidneys than in purely ischemic kidneys, but the pH in HTK-protected kidneys is higher than 6.9 for 24 hr. At 25$dGC, and especially at 35$dGC the intrarenal acidosis in Euro-Collins kidneys is much stronger than in unprotected kidneys, and the pH in HTK-protected kidneys does not decrease below 6.7. The lactate production in simple ischemic kidneys and in HTK-protected kidneys is nearly the same (80–100 $mUmol/gdw), although Euro-Collins kidneys have a steeper increase and reach higher lactate levels (330 $mUmol/gdw). The HTK solution guarantees satisfactory protection against damaging acidosis over the whole temperature range (5–35$dGC), but the Euro-Collins solution leads to a stronger and more dangerous acidosis the higher the temperature.