Sulfhydryl Reagents Prevent Dephosphorylation and Proteolysis of Histones in Isolated HeLa Metaphase Chromosomes
- 1 October 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 111 (1), 189-197
- https://doi.org/10.1111/j.1432-1033.1980.tb06092.x
Abstract
It was shown previously that histones H1 and H3, which are highly phosphorylated during mitosis in mammalian cells, become rapidly dephosphorylated during conventional metaphase chromosone isolation procedures. Dephosphorylation can be completely prevented by including SH reagents, such as p-chloromercuriphenylsulfonate or 5,5''-dithiobis(2-nitrobenzoate), in the chromosome isolation buffers. These reagents also efficiently inhibit the endogenous proteases present in isolated HeLa chromosomes and nuclei.This publication has 34 references indexed in Scilit:
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