Effect of Trypsin Treatment of Rat Adipocytes on Biological Effects and Binding of Insulin and Insulin‐Like Growth Factors
- 1 January 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 113 (3), 605-609
- https://doi.org/10.1111/j.1432-1033.1981.tb05105.x
Abstract
Trypsin-treatment of isolated rat adipocytes abolishes the metabolic effects not only of insulin, but also of the insulin-like growth factors: in trypsin-treated cells, concentrations of these hormones that are otherwise maximally effective no longer stimulate 3-O-methylglucose transport and lipogenesis or inhibit epinephrine-induced lipolysis. Concomitantly, the trypsin-treated adipocytes no longer display specific insulin binding. In contrast, the characteristics of the binding of the insulin-like growth factors are not grossly affected by prior trypsinization of the adipocytes. Apparently, the insulin-like growth factors act on glucose metabolism and antilipolysis via the insulin receptor of the adipocyte.This publication has 21 references indexed in Scilit:
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