A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1.
Open Access
- 1 October 1988
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 2 (10), 1216-1226
- https://doi.org/10.1101/gad.2.10.1216
Abstract
ATF is a cellular transcription factor involved in the regulation of multiple adenovirus E1A- and cellular cAMP-inducible promoters. Using DNA affinity chromatography, we have purified ATF and found that a series of polypeptides copurify in a sequence-specific manner. We demonstrate that these polypeptides represent a family of proteins that are related by DNA-binding specificity and by immunological cross-reactivity. This family includes the transcription factor AP-1, whose recognition sequence, GTGAGTCAA, differs from the ATF consensus, GTGACGTCAA, by the absence of a cytosine residue. Our results further indicate that there are multiple forms of both ATF and AP-1. The immunological cross-reactivity and related DNA-binding specificities suggest that ATF and AP-1 contain similar amino acid sequences and may have originated from a common gene.This publication has 41 references indexed in Scilit:
- The c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genesCell, 1988
- Expression of the SV40 promoter in fission yeast: Identification and characterization of an AP-1-like factorCell, 1988
- A human retinoic acid receptor which belongs to the family of nuclear receptorsNature, 1987
- A multiplicity of CCAAT box-binding proteinsCell, 1987
- The DNA-binding domains of the jun oncoprotein and the yeast GCN4 transcriptional activator protein are functionally homologousCell, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- PROTEIN SERINE/THREONINE KINASESAnnual Review of Biochemistry, 1987
- STEROID RECEPTOR REGULATED TRANSCRIPTION OF SPECIFIC GENES AND GENE NETWORKSAnnual Review of Genetics, 1985
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981