Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.
- 1 October 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (10), 5958-5962
- https://doi.org/10.1073/pnas.78.10.5958
Abstract
Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. This technique was used to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide and cytochalasins B and D on this parameter. The total heat evolved in the interval 10-37.degree. C scanned at 1.degree. C min-1 ranged from 300-2500 .times. 10-12 cal/cell, depending on cell density, glucose concentration and the presence or absence of various drugs.This publication has 11 references indexed in Scilit:
- Calorimetry as an Analytical Tool in Biochemistry and BiologyPublished by Wiley ,1976
- L-lactate transport in Ehrlich ascites-tumour cellsBiochemical Journal, 1976
- INHIBITION OF PHAGOCYTOSIS AND PLASMA MEMBRANE MOBILITY OF THE CULTIVATED MACROPHAGE BY CYTOCHALASIN BThe Journal of cell biology, 1974
- Cytochalasin A and B. Inhibition of sugar uptake in cultured cells.1972
- MEASUREMENT OF RATES OF PHAGOCYTOSISThe Journal of cell biology, 1969
- THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTESThe Journal of Experimental Medicine, 1965
- CYTOTOXIC TEST FOR DEMONSTRATION OF MOUSE ANTIBODY.1964
- Fluoride inhibition of sodium extrusion from swine erythrocytes and its metabolic correlatesArchives of Biochemistry and Biophysics, 1964
- The Biochemical Basis of Phagocytosis. IV. Some Aspects of Carbohydrate Metabolism During Phagocytosis*Biochemistry, 1962
- THE ENTHALPY CHANGE ON ADENOSINE TRIPHOSPHATE HYDROLYSIS. IJournal of Biological Chemistry, 1955