Bile‐Salt‐Binding Polypeptides in Plasma Membranes of Hepatocytes Revealed by Photoaffinity Labelling

Abstract

1 Photoaffinity labelling of a subfraction of plasma membranes of rat liver, enriched with sinusoidal surfaces, with the sodium salts of (3β-azido-7α, 12α-dihydroxy-5β-cholan-24-oyl)-2-amino[2-³H(N)]ethanesuifonic acid, (7, 7-azo-3α, 12α-dihydroxy-5β-cholan-24-oyl)-2-amino[2-³H(N)]ethanesulfonic acid and (11ξ-azido-12-oxo-3α, 7α-dihydroxy-5β-cholan-24-oyl)-2-amino[2-³H(N)]ethanesulfonic acid resulted with each derivative in a clear covalent incorporation of radioactivity into polypeptides with the apparent molecular weights of 67000, 52000, 48000, 43000 and about 20000. 2 Photoaffinity labelling of a membrane subfraction predominantly composed of bile canalicular membranes by the photolabile derivatives of the conjugated bile salts also showed covalent incorporation of radioactivity into polypeptides of the same apparent molecular weights as with the subfraction enriched with the sinusoidal membranes. 3 The extent of photoaffinity labelling of the different membrane polypeptides is dependent upon the photolabile bile-salt derivative used. However, with each of the photolabile derivatives the relative ratio of the labelling of the different membrane polypeptides was similar for both membrane subfractions. Provided that the uptake as well as the secretion of bile salts by hepatocytes are carrier-mediated processes, this suggests the participation of the same polypeptides in both processes.