Studies on the Isolation and Growth of Plant Rusts in Host Tissue Cultures and upon Synthetic Media. II. Uromyces ari-triphylli

Abstract

A tissue culture technique has allowed the establishment of callus cultures from corns of Arisaema triphyllum systemically infected with U. ari-triphylli, which were maintained for several years by continuous subdivision and transfer. The rust has periodically produced aecia upon these calluses. Five strains of this rust have been isolated from these dual membered cultures and grown in axenic culture in the absence of the host cells. These isolated strains have been maintained on various synthetic media, and all of them were capable of reinfecting Arisaema in tissue culture and under field conditions and of forming the characteristic sori with viable spores upon the inoculated hosts.