Acridine orange staining of the mammalian fibroblast cell coat
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 64 (2), 189-193
- https://doi.org/10.1007/bf00490098
Abstract
Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compunds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric-in vitro-measurements and enzymatic digestions, respectively.Keywords
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