Lactoferrin binds to cell membrane DNA. Association of surface DNA with an enriched population of B cells and monocytes.
Open Access
- 1 March 1983
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 71 (3), 611-618
- https://doi.org/10.1172/jci110807
Abstract
The binding of human 125I-labeled lactoferrin (LF) to a population of adherent mononuclear cells (ADMC) and nonrosetting lymphocytes (E-) was abolished by prior treatment of the cells with deoxyribonuclease (DNase), but not ribonuclease (RNase). When DNase-treated ADMC were incubated with exogenous DNA, the binding of 125I-LF was restored. Enzymatic digestion with other enzymes, trypsin, phospholipase D, and neuraminidase, did not significantly influence 125I-LF binding. Saturable binding of LF at 0 degrees C was demonstrated for both E- and ADMC, with equilibrium dissociated constants of 0.76 x 10(-6) M and 1.8 x 10(-6) M, respectively. E- cells bound 2.5 x 10(7) and ADMC bound 3.3 x 10(7) molecules of Lf at saturation. Cell membranes were isolated from ADMC, E- and E+ and reacted with 125I-labeled LF; significant binding was only seen with ADMC and E-. Prior treatment of the membranes with DNase abolished the binding. Immunofluorescence studies indicated that a population of ADMC and E-, but not E+, exhibited a peripheral staining pattern for LF. Prior treatment of ADMC and E- with DNase abolished the surface immunofluorescence. This study provides evidence that cell membrane DNA acts as a binding site for exogenous LF. This is a novel role for DNA that has not been previously reported. Furthermore, it points to a basic difference between E+ cells vs. ADMC and E- cells in respect to their possession of cell surface DNA.This publication has 26 references indexed in Scilit:
- The binding of human lactoferrin to mouse peritoneal cells.The Journal of Experimental Medicine, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Characterization of DNA excreted from phytohemagglutinin-stimulated lymphocytes.The Journal of Experimental Medicine, 1976
- SOLID-PHASE RADIOIMMUNOASSAY FOR MEASUREMENT OF LACTOFERRIN IN HUMAN-PLASMA - VARIATIONS WITH AGE, SEX, AND DISEASE1976
- Membrane Receptors and Hormone ActionAdvances in protein chemistry, 1976
- Identification of DNA-binding lymphocytes in patients with systemic lupus erythematosus.JCI Insight, 1975
- Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system.1975
- Technical Aspects of the Rosette Tests Used to Detect Human Complement Receptor (B) and Sheep Erythrocyte-Binding (T) LymphocytesThe Journal of Immunology, 1973
- Detection of endotoxin in human blood and demonstration of an inhibitor.1970
- Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.1968