Cloning a novel human brain inward rectifier potassium channel and its functional expression in Xenopus oocytes
- 18 July 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 348 (3), 239-243
- https://doi.org/10.1016/0014-5793(94)00612-1
Abstract
We have cloned a novel inward rectifier K+ channel (hIRK2) from a human frontal cortex cDNA library. The amino acid sequence of hIRK2 shares 60% and 40% identity with the mouse IRK1 and the rat ROMK1 channels, respectively. Xenopus oocytes injected with hIRK2 cRNA showed an inwardly rectifying K+ current that had a prominent ‘N-shape’ I-V curve and was blocked by extracellular Ba2+. The hIRK2 channel has two unique features: (a) an 18 amino acid insertion between the first transmembrane region and the pore, and (b) restricted mRNA distribution found only in human brain and heart.Keywords
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