Bone functional changes in intact, ovariectomized, and ovariectomized, hormone-supplemented adult cynomolgus monkeys (Macaca fascicularis) evaluated by serum markers and dynamic histomorphometry
- 1 April 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 9 (4), 527-540
- https://doi.org/10.1002/jbmr.5650090413
Abstract
Several parameters of bone mass and function were investigated in three experiments involving intact, ovariectomized, or hormone‐supplemented ovariectomized female cynomolgus monkeys. Ovariectomized animals had increased serum levels of alkaline phosphatase and acid phosphatase compared with intact and hormone‐supplemented animals. Vertebral bone mass measured ex vivo by dual‐photon absorptiometry was reduced by 11–19% in ovariectomized animals compared with intact and hormone‐supplemented animals. The most dramatic effects observed with ovariectomy were markedly increased (30–60%) bone formation rates in vertebral cancellous bone, primarily caused by higher activation frequency of basic multicellular units of bone. In addition, combined resorption and reversal periods were decreased and formation period increased in untreated ovariectomized animals. Changes in static histomorphometry parameters were less dramatic, cancellous bone volume being 1–14% lower in ovariectomized animals compared with intact or ovariectomized hormone‐supplemented animals. The data indicate that changes in bone resorption are primarily responsible for the lower bone mass of estrogen deficiency and increased bone mass in hormone‐supplemented animals. Bone changes in ovariectomized cynomolgus monkeys resemble those in women after menopause and similarly respond positively to hormone supplementation. As such, cynomolgus monkeys are an excellent model for studying the basic mechanisms of osteoporosis and for the development of suitable therapeutic regimens.Keywords
Funding Information
- the National Institutes of Health (HL33492, HL14164, AM35736, AG09827, RR00919)
This publication has 70 references indexed in Scilit:
- Perspective how many women have osteoporosis?Journal of Bone and Mineral Research, 1992
- Trabecular and endocortical bone remodeling in postmenopausal osteoporosis: Comparison with normal postmenopausal womenOsteoporosis International, 1990
- Star volume of marrow space and trabeculae of the first lumbar vertebra: Sampling efficiency and biological variationBone, 1989
- Reversal of postmenopausal vertebral bone loss by oestrogen and progestogen: a double blind placebo controlled studyBMJ, 1988
- Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committeeJournal of Bone and Mineral Research, 1987
- Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphataseJournal of Bone and Mineral Research, 1987
- Involutional OsteoporosisNew England Journal of Medicine, 1986
- A new method for the two‐dimensional analysis of bone structure in human iliac crest biopsiesJournal of Microscopy, 1986
- Ovariectomy, social status, and atherosclerosis in cynomolgus monkeys.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1985
- Reconstruction of the resorptive site in iliac trabecular bone: A kinetic model for bone resorption in 20 normal individualsMetabolic Bone Disease and Related Research, 1984