PSEUDOMONAS-AERUGINOSA PHAGES WHICH DNA IS SIMILAR IN STRUCTURE WITH MU1 PHAGE DNA .2. THE EVIDENCE OF RELATIONSHIP OF D3112, B3 AND B39 PHAGES - ANALYSIS OF RESTRICTION WITH ENDONUCLEASES, ISOLATION OF RECOMBINANT BETWEEN D3112 AND B3 PHAGES

Abstract

It is found that bacteriophages B3 and B39 specific for P. aeruginosa have the same genome structure as previously described phage D3112. On the right (S) end of their genomes, a variable non-phage DNA is located (approximately 0.9-2.5 kilobases for different phages). This variable DNA probably has its origin from different regions of bacterial chromosome. In genome of 1 of the phages, B3'' phage, such variable DNA (not more than 150 base pairs) was found on the left end of DNA molecule. Isolation of a viable B3 .times. D3112 recombinant phage and analysis of its genome with restriction technique and with studies of homo- and heteroduplex molecules confirmed the genetical relationship of B3 and D3112. Some essential non-homology of B3 and D3112 DNA were found on the right ends of genomes of the phages.