Expression of ricin A chain in Escherichia coli
- 25 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 216 (1), 73-78
- https://doi.org/10.1016/0014-5793(87)80759-7
Abstract
DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.Keywords
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