Notizen: Über die Stäbchendoppelbrechung von Myofibrillen in vitro bei wechselnden Spannungen im Kulturmedium

Abstract

Measurements were made of rod double refraction in myofibrils of femur and tibia muscles of Calliphora erythrocephala. By comparison of imbibition expts. with myofibrils from cultures of different degrees of refraction, a group of curves of the same or nearly the same base was obtained. For the steeper portions of the curves a close approximation of [delta]2 to 0.5 must be assumed, and the increasing flatness of the curves is the expression of varying contraction of the interstices of the mixed substances. If the degree of swelling is placed approx. [delta] 2=0, a hypothetical value of [delta]2=0.06 for the flattest part of the curve can be calculated from the tabulated values for *''q", and for the group of curves the dependence of rod double refraction on the relative volumes [delta]1[delta]2 can be detd. The increase in the intensity of double refraction is not linked, as is usual in polarization microscopy, with increasing degree of orientation, but with the approximation to equivalence of volumes [delta]1[delta]2 of the mixed substances.