Nuclear magnetic resonance monitoring of sodium in biological tissues

Abstract

In recent years, the ²³Na nuclear magnetic resonance (NMR) technique has been applied to the study of biological tissues. The advantages of this method are noninvasiveness and good sensitivity. The resonances of the intra- and extra-cellular sodium can be separated by the addition of shift reagents to the extracellular compartment. The method has been mostly applied to cell suspensions, kidney tubules, glands, and small organs. Owing to line-broadening effects, the NMR visibility of the intracellular sodium is reduced to 40% in most cases but can be lower or higher. Time-dependent measurements are possible with adequate life-supporting equipment, allowing the determination of transport parameters. ²³Na relaxation times are short in tissues (below 50 ms) and highly dependent on the medium composition. The application of the ²³Na NMR technique to intact organs can be hampered by the difficulty of getting a good distribution of the shift reagent in the extracellular milieu. A summary of the studies performed is presented with specific examples to illustrate typical applications.Key words: ²³Na nuclear magnetic resonance, shift reagents, sodium quantitation, sodium transport, ²³Na relaxation times.