Characterization of a candidate bcl-1 gene.
Open Access
- 1 October 1991
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 11 (10), 4846-4853
- https://doi.org/10.1128/mcb.11.10.4846
Abstract
The t(11;14)(q13;q32) translocation has been associated with human B-lymphocytic malignancy. Several examples of this translocation have been cloned, documenting that this abnormality joins the immunoglobulin heavy-chain gene to the bcl-1 locus on chromosome 11. However, the identification of the bcl-1 gene, a putative dominant oncogene, has been elusive. In this work, we have isolated genomic clones covering 120 kb of the bcl-1 locus. Probes from the region of an HpaII-tiny-fragment island identified a candidate bcl-1 gene. cDNAs representing the bcl-1 mRNA were cloned from three cell lines, two with the translocation. The deduced amino acid sequence from these clones showed bcl-1 to be a member of the cyclin gene family. In addition, our analysis of expression of bcl-1 in an extensive panel of human cell lines showed it to be widely expressed except in lymphoid or myeloid lineages. This observation may provide a molecular basis for distinct modes of cell cycle control in different mammalian tissues. Activation of the bcl-1 gene may be oncogenic by directly altering progression through the cell cycle.Keywords
This publication has 48 references indexed in Scilit:
- Break in the BCL1 locus is closely associated with intermediate lymphocytic lymphoma subtypeGenes, Chromosomes and Cancer, 1990
- Transcripts of one of two Drosophila cyclin genes become localized in pole cells during embryogenesisNature, 1989
- At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cellsJournal of Molecular Biology, 1987
- The Role of Chromosomal Translocations in B- and T-Cell NeoplasiaAnnual Review of Immunology, 1987
- Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocationCell, 1986
- Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around Jh on chromosome 14 and near a transcriptional unit on 18Cell, 1985
- Evolution of tumours and the impact of molecular oncologyNature, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy-chain locusNature, 1984
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983