Abdominal Vagotomy Delays the Onset of Puberty and Inhibits Ovarian Function in the Female Rat
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in Neuroendocrinology
- Vol. 36 (4), 261-267
- https://doi.org/10.1159/000123465
Abstract
To examine the influence that the vagus nerve may have on ovarian development and on the timing of puberty, both anterior and posterior vagi were sectioned at the abdominal level in 24-day-old female rats. Abdominal vagotomy (AV) resulted in a marked delay in the onset of puberty, as measured by the age at vaginal opening and at first diestrus. No differences in circulating levels of LH, FSH, PRL, TSH or GH between control and vagotomized rats were observed between the time of AV and vaginal opening. Nevertheless, estradiol (E2) and progesterone (P) response to human chorionic gonadotropin (hCG) in vitro was profoundly depressed in ovaries of vagotomized rats. This decreased ovarian secretory activity was further reflected in vivo by a reduced ovarian weight and, more prominently, by a reduced uterine weight. Ovarian release of androgens, however, was increased in vagotomized rats suggesting an enhanced activity of the thecal-interstitial gland compartment of the ovary. The reduced E2 and P response to hCG could not be attributed to changes in hCG-LH or FSH receptor number. Likewise, the inhibitory effect of AV on the ovary was not due to changes in ovarian β-adrenergic receptor content. Concentration of LHRH binding sites in granulosa cells was increased after AV. However, since LHRH receptor content decreases with ovarian maturation, this change may simply reflect delayed ovarian development rather than being the actual factor mediating the effect of AV. The delay in puberty induced by AV was not due to interference with nutrition of the animal, because at no age examined was the body weight of vagotomized rats different from that of controls. In addition, the effect of AV was not due to a decreased availability of cholesterol to the ovary, because levels of high- and low-density lipoproteins were normal in vagotomized rats. It is concluded that AV delays the onset of puberty and depresses ovarian function by mechanisms which do not involve alterations in ovarian gonadotropin or β-adrenergic receptors, changes in serum levels of pituitary hormones or availability of precursors for steroid synthesis. The possibility arises that the vagal control of the ovary is, instead, exerted by peptidergic fibers known to be contained in the abdominal vagus.Keywords
This publication has 22 references indexed in Scilit:
- Hyperprolactinemia-Induced Precocious Puberty in the Female Rat: Ovarian Site of Action*Endocrinology, 1978
- Effect of abdominal vagotomy of the pregnant rat on LH and progesterone concentrations and fetal resorptionReproduction, 1978
- The occurrence of somatostatin-like immunoreactivity in the vagal nervesActa Physiologica Scandinavica, 1978
- Hormonal Control of 17β-Estradiol Biosynthesis in Proestrous Rat Follicles: Estradiol Production by Isolated Theca Versus Granulosa*Endocrinology, 1978
- Serum LH and FSH following Passive Immunization Against Circulating Testosterone in the Intact Male Rat and in Orchidectomized Rats Bearing Subcutaneous Silastic Implants of TestosteroneArchives of Andrology, 1978
- Heptadecapeptide gastrin in the vagal nerve.Proceedings of the National Academy of Sciences, 1977
- The Role of Estrogen in the Regulation of Luteal Progesterone Secretion in the Rat After Day 12 of Pregnancy1Endocrinology, 1977
- Vasoactive Intestinal Polypeptide: Abundant Immunoreactivity in Neural Cell Lines and Normal Nervous TissueScience, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956