Identification of Malaria-Infected Mosquitoes by a Two-Site Enzyme-Linked Immunosorbent Assay
- 1 March 1984
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 33 (2), 227-231
- https://doi.org/10.4269/ajtmh.1984.33.227
Abstract
A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two-site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.This publication has 1 reference indexed in Scilit:
- Monoclonal antibodies identify the protective antigens of sporozoites of Plasmodium knowlesi.Proceedings of the National Academy of Sciences, 1982