Abstract
The effect of antibiotics on the binding of phenylalanyl-oligonucleotide to ribosomes has been examined. The results show that many classes of antibiotics can interfere with binding of the aminoacyl-oligonucleotide terminus of tRNA to ribosomes: chloramphenicol, sparsomycin, D-WIN-5094, vernamycin A, PA114A, streptogramin, amicetin, gougerotin, tylosin, and spiramycin III. The results are consistent with the hypothesis that these antibiotics inhibit protein synthesis by interfering with the binding of the aminoacyl-end of aminoacyl-tRNA to ribosomes.

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