ISOLATION OF COLPIDIUM STRIATUM STOKES IN BACTERIA-FREE CULTURES AND THE RELATION OF GROWTH TO pH OF THE MEDIUM

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Abstract
The method used in isolating Colpidium striatum in bacteria-free cultures is outlined, and suitable culture media are described. Growth of the ciliates was found to occur within the pH range 4.0 to 8.6. Within this range two growth "maxima" were observed, one above and one below pH 7.0. Addition of sodium acetate to the medium restricted the growth range, depressed the fission rate, and brought about a replacement of the two growth "maxima" by a single maximum. Division rate was found to vary with the pH of the medium. Acid fermentation of dextrose, liquefaction of gelatin, and peptonization of litmus milk were observed.