PROTEOLYTIC ATTACK OF THE CHROMOPROTEINS OF PORPHYRA BY MARINE BACTERIAL ENZYMES
- 1 October 1964
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 88 (4), 974-+
- https://doi.org/10.1128/jb.88.4.974-980.1964
Abstract
Three chromoproteins were isolated from the marine red alga, Porphyra leucosticta (Thuret), and were purified by adsorption chromatography on calcium phosphate gel and by ammonium sulfate fractionation. The algal chromoproteins were subjected to the proteolytic action of cultures of actively growing marine bacteria, marine bacterial culture filtrates, trypsin, and various marine bacterial enzyme preparations. Digestion of the chromoproteins was followed through various incubation intervals by measuring the loss of chromophore color and the increase in either ninhydrin-positive material (570 m[mu]) or 280-m[mu] light-absorbing material. There was good correlation between the loss of color and enzymatic hydrolysis of the proteins. This was particularly evident when a marine pseudomonad proteinase preparation was used as the proteolytic agent. In every digestion mixture, phycoerythrin was more resistant to degradation than was phycocyanin or allophycocyanin. Phycoerythrin was also more resistant to heat than was phycocyanin or allophycocyanin.Keywords
This publication has 14 references indexed in Scilit:
- Studies on Chromoproteins in Japanese Nori, Porphyra teneraThe Journal of Biochemistry, 1961
- A Possible Function of Phycoerythrin in Intertidal Red AlgaeNature, 1960
- Some atypical algal chromoproteinsBiochimica et Biophysica Acta, 1960
- Some Characteristics of Proteolytic System of a Marine Bacterial Species.Experimental Biology and Medicine, 1960
- [Not Available].1958
- Comparative biochemical studies of the phycobilinsArchives of Biochemistry and Biophysics, 1958
- STUDIES ON CHROMOPROTEINS IN JAPANESE NORI (PORPHYRA TENERA)The Journal of Biochemistry, 1957
- Comparative studies of chromatographically separated phycoerythrins and phycocyaninsArchives of Biochemistry and Biophysics, 1955
- Tricalcium phosphate as an adsorbent in the chromatography of proteinsBiochemical Journal, 1951
- PHOTOSYNTHETIC ACTION SPECTRA OF MARINE ALGAEThe Journal of general physiology, 1950