Estimation of 2-Aminofluorene and Related Compounds in Biologic Material

Abstract

A method for the estimation of 2-aminofluorene in biologic material is presented. The method depends on diazotization of the amino group in an acid medium, followed by coupling with sodium 2-naphthol-3,6-disulfonate in strongly alkaline soln. By the hydrolysis of N-acetyl-2-aminofluorene and N,N-diacetyl-2-aminofluorene, these 2 compounds may likewise be estimated. The method is applicable to 2-nitrofluorene following its reduction. The Boniger reaction for detection of primary amines using sodium l,2-naphthoquinone-4-sulfate is applicable to 2-aminofluorene and under suitable conditions may be used for its quantitative estimation. The Janovsky reaction may be applied to 2-nitrofluorene in acetone soln. The use of these methods enables the approx. solubility in H2O of the 4 fluo-rene derivatives listed to be estimated, and these data are included. The application of these methods to biologic material is illustrated.