Circulating human T and B lymphocytes express the p55 interleukin‐2 receptor molecule (TAC, CD25)

Abstract

Using a highly sensitive immunofluorescence procedure 15‐45% of blood lymphocytes from normal human donors can be shown lo react with anti‐TAC (CD25) monoclonal antibodies. The standard procedure for indirect immunofluorescencc docs not allow the detection of these CD25‐positive cells. Ten healthy adults were tested, and all showed a population of positive cells. Three donors have been tested more than once, and have given consistent results. Three different CD25 antibodies were used, again with consistent results. Double‐marker studies showed that the positive cells included B cells, as well as CD4‐bearing and CD8‐bearing T cells.