Mutagenicity to Mammalian Cells of Epoxides and Other Derivatives of Polycyclic Hydrocarbons
- 1 December 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (12), 3195-3199
- https://doi.org/10.1073/pnas.68.12.3195
Abstract
The cytotoxicity and mutagenicity of several polycyclic hydrocarbons and their K-region derivatives were tested in a clone of Chinese hamster cells; the production of clones resistant to 8-azaguanine was used as the marker for mutagenesis. In the series related to benz(a)anthracene, the K-region epoxide was highly mutagenic, and the phenol was less mutagenic; the hydrocarbon and cis and trans-dihydrodiols were not mutagenic. Seven resistant clones were isolated and retained their drug-resistance; three of these could be reverted to the wild type. There was no difference in the chromosome numbers among the parent and mutant clones. The results in the methylcholanthrene series were similar to those for benz(a)anthracene. However, in the dibenz(a,h)anthracene series, the phenol was more mutagenic than the epoxide. 7-Methylbenz(a)anthracene epoxide and 7-bromomethylbenz(a)anthracene were highly mutagenic, 7-bromomethyl-12-methylbenz(a)anthracene was less mutagenic, and the parent hydrocarbons were inactive. These results demonstrate that metabolic activation of polycyclic hydrocarbon is required for mutagenic activity in mammalian cells.Keywords
This publication has 19 references indexed in Scilit:
- Determination of the mutagenic activity to bacteriophage T4 of carcinogenic and noncarcinogenic compounds.1970
- Diploid Azaguanine-Resistant Mutants of Cultured Human FibroblastsScience, 1970
- Interactions of the k-region epoxides of phenanthrene and dibenz[a,h]anthracene with nucleic acids and histoneBiochemical Pharmacology, 1970
- Carcinogenesis by chemicals: an overview--G. H. A. Clowes memorial lecture.1970
- The metabolism of some aromatic hydrocarbons by mouse embryo cell culturesBiochemical Pharmacology, 1970
- Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agentsJournal of Cellular Physiology, 1969
- Theory of tumour initiation by chemical carcinogens: Dependence of activity on structure of ultimate carcinogenEuropean Journal of Cancer (1965), 1968
- Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proceedings of the National Academy of Sciences, 1968
- A METHOD OF SELECTING FOR AUXOTROPHIC MUTANTS OF HELA CELLSThe Journal of Experimental Medicine, 1960
- CLONAL GROWTH OF MAMMALIAN CELLS IN VITROThe Journal of Experimental Medicine, 1956