Feulgen Cytophotometry of Pine Nuclei. II. Effect of Pectinase Used in Cell Sfparation
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 54 (4), 201-204
- https://doi.org/10.3109/10520297909112683
Abstract
Pectinase used for cell separation prior to cytophotometry contains a DNase that is able to penetrate the cells of pine [Pinus rigida and P. lambertiana] root tips and attack nuclear DNA. When pine root tips were exposed to 1% pectinase (pH 6.0), there was a decrease in nuclear DNA content at every sample point and a sharp drop between 16-20 h. The effect of the DNase was eliminated by preparing the enzyme solution in 0.01 M sodium citrate or 0.001 M EDTA. Heat denaturation of the DNase should also be effective and might be used in combination with the Mg chelators.This publication has 10 references indexed in Scilit:
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