MIXED HEART CELL-LYMPHOCYTE REACTIONS AND GRAFT SURVIVALS IN AG-B-COMPATIBLE RATS1
- 1 January 1977
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 23 (1), 53-59
- https://doi.org/10.1097/00007890-197701000-00010
Abstract
A method of dissociation of the rat heart cell for the mixed lymphocyte culture purpose is described. The treatment of the young rat heart with 0.1% collagenase-hyaluronidase solution yielded satisfactory heart cell suspension. The heart cells, thus obtained after mitomycin C treatment but not irradiation, stimulated allogeneic lymphocytes in the presence of 2-mercaptoethanol. In the Fischer to Lewis combination compatible at the Ag-B locus, strong reactions of Lewis lymphocytes to the dissociated heart and skin cells of the Fischer rat were related to the acute rejection of heart and skin grafts, while negative reactions by the kidney and spleen cells reflected a prolonged survival of the kidney graft. The role of skin- and heart-specific antigens in the rejection phenomenon is discussed.This publication has 6 references indexed in Scilit:
- The use of freshly explanted mouse epidermal cells for the in vitro induction and detection of cell-mediated cytotoxicityCellular Immunology, 1976
- DIFFERENTIAL SURVIVALS OF F1 HYBRID ALLOGRAFTS IN PARENTAL RECIPIENTSTransplantation, 1976
- ALLOGRAFT IMMUNITY TO HISTOCOMPATIBILITY AND ORGAN-SPECIFIC ANTIGENSTransplantation, 1976
- In vitro studies on single beating rat heart cellsExperimental Cell Research, 1963
- EARLIER STUDIES OF BLOOD GROUPS IN THE RATAnnals of the New York Academy of Sciences, 1962
- Blood Groups of the Rat (Rattus Norvegicus) and Their InheritanceProceedings of the National Academy of Sciences, 1947