Evolutionary Conservation of Chloroplast Genes Coding for the Large Subunits of Fraction 1 Protein

Abstract
Crystalline fraction 1 protein, obtained from 4 spp. of Nicotiana, have identical polypeptide compositions and isolelectric points. The tryptic peptide map of the large subunit of this protein from N. knightiana and N. paniculata differs from that of N. tomentosa and N. tomentosiformis. Since the large subunits of fraction 1 protein are coded by chloroplast DNA, the difference in their primary structure reflects the structural changes of the chloroplast genes containing the coding information. Apparently the rate of mutation of chloroplast DNA seems to be higher than predicted from the analysis of isoelectric points of this protein.